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cell culture schematic design image  (ibidi GmbH)

 
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    ibidi GmbH cell culture schematic design image
    A, <t>schematic</t> diagram and illustration of experimental protocol 1. Human aortic endothelial cells (HAECs) were seeded and exposed to low shear stress conditions (3 dynes cm−2) for 40 h to promote <t>cell</t> alignment. Cells were then either exposed to increased shear stress (20 dynes cm−2) or maintained at control conditions (3 dynes cm−2) for 1 h. Following a 30 min recovery period (3 dynes cm−2) designed to mimic subsequent protocols, cells were stimulated with insulin or vehicle for 30 min and fixed in their chambers. Immunofluorescence and <t>image</t> analysis were performed in all conditions. The cell image displayed shows fluorescence associated with stains for phospho‐eNOS (green) and 4′,6‐diamidino‐2‐phenylindole for nuclei (blue). B, the change in the ratio of phospho‐eNOS/phospho‐MAPK upon insulin stimulation in cells previously exposed to control conditions vs. increased shear stress. The cartoon underneath further illustrates the results. IR, insulin receptor. n = 4/condition. Values are means ± SEM. * P < 0.05 vs. control condition.
    Cell Culture Schematic Design Image, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+schematic+design+image/pmc06312413-503-1-9?v=ibidi+GmbH
    Average 90 stars, based on 1 article reviews
    cell culture schematic design image - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Increased endothelial shear stress improves insulin‐stimulated vasodilatation in skeletal muscle"

    Article Title: Increased endothelial shear stress improves insulin‐stimulated vasodilatation in skeletal muscle

    Journal: The Journal of Physiology

    doi: 10.1113/JP277050

    A, schematic diagram and illustration of experimental protocol 1. Human aortic endothelial cells (HAECs) were seeded and exposed to low shear stress conditions (3 dynes cm−2) for 40 h to promote cell alignment. Cells were then either exposed to increased shear stress (20 dynes cm−2) or maintained at control conditions (3 dynes cm−2) for 1 h. Following a 30 min recovery period (3 dynes cm−2) designed to mimic subsequent protocols, cells were stimulated with insulin or vehicle for 30 min and fixed in their chambers. Immunofluorescence and image analysis were performed in all conditions. The cell image displayed shows fluorescence associated with stains for phospho‐eNOS (green) and 4′,6‐diamidino‐2‐phenylindole for nuclei (blue). B, the change in the ratio of phospho‐eNOS/phospho‐MAPK upon insulin stimulation in cells previously exposed to control conditions vs. increased shear stress. The cartoon underneath further illustrates the results. IR, insulin receptor. n = 4/condition. Values are means ± SEM. * P < 0.05 vs. control condition.
    Figure Legend Snippet: A, schematic diagram and illustration of experimental protocol 1. Human aortic endothelial cells (HAECs) were seeded and exposed to low shear stress conditions (3 dynes cm−2) for 40 h to promote cell alignment. Cells were then either exposed to increased shear stress (20 dynes cm−2) or maintained at control conditions (3 dynes cm−2) for 1 h. Following a 30 min recovery period (3 dynes cm−2) designed to mimic subsequent protocols, cells were stimulated with insulin or vehicle for 30 min and fixed in their chambers. Immunofluorescence and image analysis were performed in all conditions. The cell image displayed shows fluorescence associated with stains for phospho‐eNOS (green) and 4′,6‐diamidino‐2‐phenylindole for nuclei (blue). B, the change in the ratio of phospho‐eNOS/phospho‐MAPK upon insulin stimulation in cells previously exposed to control conditions vs. increased shear stress. The cartoon underneath further illustrates the results. IR, insulin receptor. n = 4/condition. Values are means ± SEM. * P < 0.05 vs. control condition.

    Techniques Used: Shear, Control, Immunofluorescence, Fluorescence



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    cell culture schematic design image  (ibidi GmbH)
    90
    ibidi GmbH cell culture schematic design image
    A, <t>schematic</t> diagram and illustration of experimental protocol 1. Human aortic endothelial cells (HAECs) were seeded and exposed to low shear stress conditions (3 dynes cm−2) for 40 h to promote <t>cell</t> alignment. Cells were then either exposed to increased shear stress (20 dynes cm−2) or maintained at control conditions (3 dynes cm−2) for 1 h. Following a 30 min recovery period (3 dynes cm−2) designed to mimic subsequent protocols, cells were stimulated with insulin or vehicle for 30 min and fixed in their chambers. Immunofluorescence and <t>image</t> analysis were performed in all conditions. The cell image displayed shows fluorescence associated with stains for phospho‐eNOS (green) and 4′,6‐diamidino‐2‐phenylindole for nuclei (blue). B, the change in the ratio of phospho‐eNOS/phospho‐MAPK upon insulin stimulation in cells previously exposed to control conditions vs. increased shear stress. The cartoon underneath further illustrates the results. IR, insulin receptor. n = 4/condition. Values are means ± SEM. * P < 0.05 vs. control condition.
    Cell Culture Schematic Design Image, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+schematic+design+image/pmc06312413-503-1-9?v=ibidi+GmbH
    Average 90 stars, based on 1 article reviews
    cell culture schematic design image - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A, schematic diagram and illustration of experimental protocol 1. Human aortic endothelial cells (HAECs) were seeded and exposed to low shear stress conditions (3 dynes cm−2) for 40 h to promote cell alignment. Cells were then either exposed to increased shear stress (20 dynes cm−2) or maintained at control conditions (3 dynes cm−2) for 1 h. Following a 30 min recovery period (3 dynes cm−2) designed to mimic subsequent protocols, cells were stimulated with insulin or vehicle for 30 min and fixed in their chambers. Immunofluorescence and image analysis were performed in all conditions. The cell image displayed shows fluorescence associated with stains for phospho‐eNOS (green) and 4′,6‐diamidino‐2‐phenylindole for nuclei (blue). B, the change in the ratio of phospho‐eNOS/phospho‐MAPK upon insulin stimulation in cells previously exposed to control conditions vs. increased shear stress. The cartoon underneath further illustrates the results. IR, insulin receptor. n = 4/condition. Values are means ± SEM. * P < 0.05 vs. control condition.

    Journal: The Journal of Physiology

    Article Title: Increased endothelial shear stress improves insulin‐stimulated vasodilatation in skeletal muscle

    doi: 10.1113/JP277050

    Figure Lengend Snippet: A, schematic diagram and illustration of experimental protocol 1. Human aortic endothelial cells (HAECs) were seeded and exposed to low shear stress conditions (3 dynes cm−2) for 40 h to promote cell alignment. Cells were then either exposed to increased shear stress (20 dynes cm−2) or maintained at control conditions (3 dynes cm−2) for 1 h. Following a 30 min recovery period (3 dynes cm−2) designed to mimic subsequent protocols, cells were stimulated with insulin or vehicle for 30 min and fixed in their chambers. Immunofluorescence and image analysis were performed in all conditions. The cell image displayed shows fluorescence associated with stains for phospho‐eNOS (green) and 4′,6‐diamidino‐2‐phenylindole for nuclei (blue). B, the change in the ratio of phospho‐eNOS/phospho‐MAPK upon insulin stimulation in cells previously exposed to control conditions vs. increased shear stress. The cartoon underneath further illustrates the results. IR, insulin receptor. n = 4/condition. Values are means ± SEM. * P < 0.05 vs. control condition.

    Article Snippet: The cell culture schematic design image is courtesy of ibidi GmbH.

    Techniques: Shear, Control, Immunofluorescence, Fluorescence